The pre-assembled Closed System of Centrifuge Tube is convenient for aseptic sampling and transfer during biological production procedures. Each is double-packed and equipped with a seal cap which can be used to substitute the pipes in case of subsequent centrifugation and analysis. The pre-assembled Closed System of Centrifuge Tube is convenient for customers in biomedical/biopharmaceutical/vaccine production industries to aseptically sample and transfer the tissue and cell culture/ vaccine solutions, which can effectively save the assembling and sampling time and lower the cost.